Cell Line Authentication DNA Test:
Authentication of a cell line is the sum of the process by which a line’s identity is verified and shown to be free of contamination from other cell lines and microbes. Using standardized techniques (Confirm Cell Line Identity Authentication DNA test), authentication enables communication among all users about the resource and ensures valid, reproducible experimental results. Obtaining and using low-passage cell lines from ATCC is a sure way to work and publish with confidence.
Verifying Cell Lines through Authentication:
Experimental success corresponds directly to the quality and conditions of the cell lines used. Cells that are kept too long in culture and are not periodically tested for genotypic or phenotypic stability may no longer be reliable models of the original source material.
This authenticates cell lines routinely with the following tests:
- Short Tandem Repeat (STR) Profiling
- Cellular Morphology
- Cytochrome C Oxidase I (COI) Assay Testing
Confirm Cell Line Identity Authentication DNA test:
Cell line authentication is still poorly reported, despite the prevalence of misidentification, the calls for community action, and the estimates of wasted research dollars. Unlike the situation for human cell lines, authentication of nonhuman cell lines is less clear and is burdened by the immature state of development of kits, testing services, databases, and accepted standards for STR or other DNA markers.